Histograms showing hydrodynamic
size distribution of 5 nm AuNPs at 4, 12, and 24 h time points from
top to bottom. Size measurements of AuNP solutions were carried out
in the presence of serum proteins at RT and 37 °C and were continuously
measured for 24 h. Data shows absolutely no change in hydrodynamic
size when measured in serum at RT (4 h, 10 nm; 12 h, 10 nm; 24 h,
10 nm), whereas there was an observable change in the AuNP size when
measured in serum (4 h, 19 nm; 12 h, 18 nm; 24 h, 18 nm) as well as
a broad size distribution.