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. 2014 Jul 24;15(8):13135–13150. doi: 10.3390/ijms150813135

Figure 5.

Figure 5

Altered expressions of MMPs and apoptosis in the postnatal TMJ of Wnt1-Cre; pMes-stop Shox2 mice. (A,B) In situ zymography on coronal sections of wild type (A) and Wnt1-Cre; pMes-stop Shox2 (B) TMJ shows MMPs activity. The ectopic MMPs activity in the glenoid fossa (arrow heads) of the Wnt1-Cre; pMes-stop Shox2 TMJ; TUNEL assay on coronal sections of P0 wild type (C) and Wnt1-Cre; pMes-stop Shox2 (D) TMJ shows an increase of apoptotic cells in the glenoid fossa of the Wnt1-Cre; pMes-stop Shox2 TMJ. Arrow heads in (C,D) point to the apoptotic cells in the glenoid fossa; (E–L) Immunohistochemical staining on coronal sections of wild type (E,G,I,K) and Wnt1-Cre; pMes-stop Shox2 (F,H,J,L) shows expressions of MMP9 (E–H) and MMP13 (I–L). Arrow heads in (E–H,K,L) point to the protein levels of MMP9 and MMP13in the condyle and glenoid fossa where expression is altered; (M) Comparison of numbers of apoptotic cells in the glenoid fossa between wild type and Wnt1-Cre; pMes-stop Shox2 mice. Standard deviation is shown as error bars. * p < 0.05.