Table 2.
Primer sets used for quantitative real-time PCR.
| Target Gene | Locus No. | Sequence of Primers | Product Size |
|---|---|---|---|
| Vasa | S75275 | F: 5'-GCGAGACTACATCTACAAC-3' | 135 bp |
| R: 5'-GAGTATCTTCACAGTCATTA-3' | |||
| SMAD1 | AF067727 | F: 5'-CTCATGTCATTTATTGCCG-3' | 138 bp |
| R: 5'-CTCGCTTATAGTGGTAGGGA-3' | |||
| Stella | BK001414 | F: 5'-CTATCATCGTCGTCAAAGG-3' | 177 bp |
| R: 5'-CTCTGCTCAATCCGAACAA-3' | |||
| Dazl | NM_001025742 | F: 5'-CGACGAAATCGGGAAGCTC-3' | 94 bp |
| R: 5'-CACAACCTCACCATACTGGGAAA-3' | |||
| GCNF | AJ783965 | F: 5'-CAACTGAACAAGCGGTATT-3' | 114 bp |
| R: 5'-GATGTATCGGATCTCTGGC-3' | |||
| c-kit | NM_022264 | F: 5'-TGCCCGAAACAAGTCATCTCC-3' | 112 bp |
| R: 5'-GGCTGAGGGTTCAACTTTATCCA-3' | |||
| β-actin | NM_031144 | F: 5'-GCTCGTCGTCGACAACGGCTC-3' | 353 bp |
| R: 5'-CAAACATGATCTGGGTCATCTTCTC-3' |