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Effects of AC activators on activation of Ciona sperm motility. The percentage of motile sperm was measured 3 min (A) or 1 min (B,C) after the addition of sperm to the solutions containing forskolin (A), NaHCO3 (B,C) with or without sAC inhibitors. The concentration of KH-7 or nigericin was 10 or 5 μM, respectively. Values are means ± SE of the results from three experiments.
