Figure 1.

The expression level of phosphoinositide-specific phospholipase γ1 (PLCγ1) in normal articular and OA chondrocytes. Normal samples were obtained from 3 patients with amputation from accident, and OA samples were obtained from 20 patients with advanced OA. (A) The protein expression levels of PLCγ1 and p-PLCγ1 in cultured normal and OA chondrocytes were detected with western blotting analysis using rat anti-PLC-γ1, p-PLC-γ1, and GAPDH antibodies according to Materials and Methods. The values represent the mean ±S.E.M. of five independent experiments, each yielding similar results (* p < 0.05); (B) The protein expression level of PLCγ1 in normal articular and OA cartilage was detected with immunohistochemistry analysis according to Materials and Method (original magnification ×100 or ×400, * p < 0.05).