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. 2014 Jul 28;15(8):13236–13246. doi: 10.3390/ijms150813236

Figure 3.

Figure 3

PLC-γ1/IP3/Ca(2+)/CaMK II axis regulates the ECM synthesis of human OA chondrocytes. OA chondrocytes were obtained from 16 patients with advanced OA. (A) Cultured cells were treated with or without U73122 (5 μM), U73343 (5 μM), R59949 (10 μM), KN93 (20 μM), and BAPTA/AM (10 μM) for 3 h, respectively. The protein expression levels of MMP-13 and Col II were detected by western blotting analysis using rat anti-MMP-13, Col II, and GAPDH antibodies; (B) Cultured cells were treated with or without U73122 (5 μM), KN93 (20 μM), and BAPTA/AM (10 μM) for 3 h, respectively. The proteinexpression levels of mTOR, p-mTOR, p-p70S6K, p-S6, Sox-9, and NF-κB p65 were detected by western blotting analysis using rat anti-mTOR, p-mTOR, p-p70S6K, p-S6, Sox-9, NF-κB p65, and GAPDH antibodies. The values represent the mean ± S.E.M. of three to five independent experiments, each yielding similar results (* p < 0.05, ** p <0.01, *** p <0.001).