Figure 2.
TCF2 inhibits the ROS activation stimulated by high concentrations of PA. ROS generation in INS-1 β-cells was analyzed after treatment with a concentration gradient of PA for 24 h. ROS generation in INS-1 β-cells treated with PA (800 μM) and overexpressing TCF2 was also analyzed (A); Expression of GCLc and GCLm was detected by Western blotting (B) in INS-1 cells overexpressing TCF2 or not; Levels in cells cultured in DMEM containing 25 mM glucose (control) or 800 μM PA were quantified relative to β-actin (C). * p < 0.05.