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. 2014 Jul 30;15(8):13317–13332. doi: 10.3390/ijms150813317

Figure 4.

Figure 4

INS-1 cells were cultured in DMEM with 6 mM or 25 mM glucose and pretreated with a concentration gradient of PA to investigate the effect of FFAs on glucose-stimulated insulin secretion (GSIS) (A); INS-1 cells pretreated with 6 or 25 mM glucose were cultured in DMEM with PA (800 μM). INS-1 cells with the control vector (blank control) and INS-1 overexpressing TCF2 were also cultured in DMEM with PA (800 μM) for 24 h. Then insulin secretion was detected to confirm the protective effect of TCF2 on GSIS (B); In addition, the expression of GLUT2 and PDX1 was evaluated by Western blotting (C) and quantified (D). * p < 0.05. NS means no statistically significant.