Celastrol reduced edema and hyperalgesia in the carrageenan-induced inflammatory pain model. The effect of vehicle (VEH, 5% PEG/5% Tween-80 in saline, 10 mL/kg, i.p.) and celastrol (CEL, 0.3 mg/kg, i.p.) on carrageenan-induced edema (A) and pain hypersensitivity (B). ***
p< 0.001 vs. non-carrageenan injection (CT), ## p < 0.01 vs. VEH; one-way ANOVA followed by Bonferroni’s multiple comparison test, n = 5–6. The time- and dose-dependent effect of celastrol on carrageenan-induced edema (C) and pain hypersensitivity (D). *
p < 0.05, **
p < 0.01, ***
p < 0.001 vs. vehicle, two-way ANOVA with Bonferroni’s post-tests, n = 5–6/group.