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. 2014 Aug 8;15(8):13738–13754. doi: 10.3390/ijms150813738

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Bone morphogenetic protein 4 (BMP4) and its receptor (BMPR1A and BMPR2) mRNA and protein expression in pulmonary arteries. (A–C) Quantitative real-time polymerase chain reaction (qPCR) was performed to analyze the BMP4 and its receptor (BMPR1A and BMPR2) expression. Expression levels of target genes were normalized to the β-actin mRNA level using the 2^−ΔΔCt method; (D–G) Western blot analysis of the BMP4 and its receptor (BMPR1A and BMPR2) protein expression; (H,I) AKT protein expression in rat pulmonary arteries homogenates from normoxic and hypoxic rats. All values are denoted as the mean ± SEM from at least three separate experiments. * p < 0.05.