Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 8;15(8):13738–13754. doi: 10.3390/ijms150813738

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Effects of BMP4 on activation of Smad1/5/8. (A) Cells were fixed and stained with anti-p-Smad1/5/8 and the nucleus was staining with 4',6-diamidino-2-phenylindole (DAPI). The phosphorylation of Smad1/5/8 was activated by BMP4 treatment in rat PASMCs, but the effect was eliminated by PI3K/AKT inhibitors, LY294002 and wortmannin. Scale bar = 10 µm; (B,C) Incubation of PASMCs with BMP4 led to phosphorylation of Smad1/5/8. The phosphorylation of Smad1/5/8 activated by BMP4 was partly inhibited by LY294002 and wortmannin (PI3K/AKT inhibitors). All values are denoted as means ± SEM from three or more independent batches of cells. “LY” means LY294002; “W” means wortmannin. * p < 0.05.