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. 2014 Aug 11;15(8):13916–13931. doi: 10.3390/ijms150813916

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Cytochrome P450 1A1 (CYP1A1) mRNA expression is differentially induced by the aryl hydrocarbon receptor (AhR) agonist 3,3',4,4',5-pentachlorobiphenyl (PCB 126) in HepG2 and HeLa cells. HepG2 and HeLa were treated with 3 µM PCB 126 for 6 h. CYP1A1 mRNA expression was determined by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Analysis was performed with normalization to ribosomal protein, large, P0 (RPLP0) mRNA. RNA expression levels significantly (p < 0.05) greater than untreated controls are indicated by an asterisk (*). RNA expression levels significantly (p < 0.05) different between PCB 126 treated HepG2 cells and PCB 126 treated HeLa cells are indicated by a number sign (#). Error bars = SEM; n = 3.