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Chromatin accessibility along the CYP1A1 promoter in HepG2 and HeLa cells. Nuclei of HepG2 cells (a) and HeLa cells (b) were isolated and digested with DNase. qRT-PCR was used to amplify two different regions of the CYP1A1 promoter that were also analyzed by bisulfite sequencing. Amplification of GAPDH was used as a positive control for accessible chromatin. Results are depicted as magnitude of the signal (∆Rn) vs. cycle number.
