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Effect of SP on alkaline phosphatase (ALP) staining and ALP activity. (A) Confluent MSCs were stained using an ALP staining kit after 7 or 14 days of cultivation in osteogenic medium containing SP (0, 1, 10, 100 or 1000 ng/mL). Scale bar = 100 μm; (B,C) ALP activity was measured at 7 or 14 days. To normalize ALP activity, the amount of ALP was normalized to the amount of DNA; (B) day 7, * p < 0.05 vs. SP (0 ng/mL); and (C) day 14, * p < 0.05 vs. SP (0 ng/mL).
