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Effect of SP on mineralization. (A) Confluent MSCs were stained with Alizarin Red after 7 or 14 days of cultivation in osteogenic medium containing SP (0, 1, 10, 100 or 1000 ng/mL). Scale bar = 100 μm; (B,C) The extracellular calcium deposition was measured at 7 or 14 days. (B) day 7, * p < 0.05, vs. SP (0 ng/mL); (C) day 14, * p < 0.05, vs. SP (0 ng/mL).
