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. 2014 Aug 13;15(8):14026–14043. doi: 10.3390/ijms150814026

Figure 6.

Figure 6

Effect of PD98059 on ALP staining and ALP activity induced by SP. (A) Confluent MSCs were stained using an ALP staining kit after 7 or 14 days of cultivation in osteogenic medium containing SP (0 or 10 ng/mL), or SP (10 ng/mL) with the ERK 1/2 inhibitor, PD98059 (10 µM). Scale bar = 100 µm; (B,C) ALP activity was measured at 7 or 14 days. To normalize ALP activity, the amount of ALP was normalized to the amount of DNA. * p < 0.05, SP (0 ng/mL) vs. SP (10 ng/mL), † p < 0.05, SP (10 ng/mL) vs. SP (10 ng/mL) with PD98059 (10 µM).