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. 2014 Aug 14;15(8):14180–14190. doi: 10.3390/ijms150814180

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Expression of human embryonic stem cell (hESC) undifferentiated markers in HUES-17 cells after exposed to pulsed electromagnetic field (PEMF) at different electric field intensities. The expression of alkaline phosphatase (AP), stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 in HUES-17 cells was detected by AP staining and immunofluorescent staining after the cells were exposed to 400 pulses PEMF at electric field intensities of 50, 100, 200, 400 kV/m or sham-exposed (control group). Abbreviations: AP, alkaline phosphatase; SSEA, stage-specific embryonic antigen; PEMF, pulsed electromagnetic field.