Down-regulation of miR-16 can reverse the effect of ART on apoptosis of bladder cancer cells. The level of miR-16 was measured in T24, RT4 and SV-HUC-1 cells. * p < 0.05, compared to SV-HUC-1 cells (A); The expression of miR-16 was detected in T24, RT4 and SV-HUC-1 cells treated with ART for 24 h. * p < 0.05, compared to vehicle (B); After transfection with miR-16 inhibitor for 24 h, T24 and RT4 cells were added with ART for an additional 24 h, and then the level of miR-16 was detected. * p < 0.05, compared to negative control (C); After transfection with miR-16 inhibitor for 24 h, T24 and RT4 cells were added with ART for an additional 48 h, and then caspase-3 activity was measured. * p < 0.05, compared to vehicle controls group. # p < 0.05, compared to negative control. $ p < 0.05, compared to negative control +ART (100 μM) treated group (D).