Figure 4.

Overexpression of Sirt3 inhibits H₂O₂-induced oxidative stress. Cortical neurons were transfected with lentivirus expressed Sirt3 (LV-Sirt3) or control lentivirus (LV-control) for 72 h, and then treated with or without 0.1 mM H₂O₂. The ROS production was measured by H₂DCFDA staining (A) and calculated (B); The mRNA levels of MnSOD and IDH2 were measured by Real-Time RT-PCR (C); The enzymatic activity of MnSOD and IDH2 was assayed (D); Scale bar: 50 μm. Data are shown as mean ± SD of five experiments. ^# p < 0.05 vs. Control. * p < 0.05 vs. LV-control.