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. 2014 Aug 21;15(8):14697–14714. doi: 10.3390/ijms150814697

Figure 7.

Figure 7

Subtilisin cleaved tubulin characterization: (A) Mass spectrum of S-tubulin showing one species of 48 kDa identified after MALDI-TOF/MS analysis. Monomer of 24 kDa is given; (B) Determination of the S-tubulin sedimentation coefficient by analytical ultracentrifugation. After extrapolation to protein zero concentration, S°20,w equal to 5.69 ± 0.05 S. In inset, the SDS-PAGE analysis of S-tubulin gives one major band at 48 kDa. Low molecular weight markers (LMW) are from top to bottom 97, 66 and 45 kDa; (C) Electron microscopy of microtubules after assembly of S-tubulin induced by taxol with a molar ratio of 1:1 in NaCl-PG buffer (i.e., 25 mM NaPi, 25 mM NaCl, 1 mM TCEP, 0.1 mM GTP, pH 7). The bar represents 2 µm.