Figure 3.

Temporal expression of erythropoietin mRNAs from blood and kidney of C.albicans-infected mice at 72 h post-infection. Transcript levels for erythropoietin were quantified by real-time reverse transcription polymerase chain reaction (RT-PCR). Bar graph represents average expression of each gene normalized to the reference genes, β-actin and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), to attain a normalized gene expression ratio. Results were means of three biological replicates and two technical replicates for each biological replicate. Bars indicate standard error of mean and * revealed significant differences at the level of p < 0.05 as compared with control group, respectively. The fold changes were calculated based on the normalization of the target gene with the average of housekeeping genes (Beta actin and GAPDH) using the ΔΔC_t method in infected mice as compared to that in uninfected mice.