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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Placenta. 2007 Jan 10;28(0):912–919. doi: 10.1016/j.placenta.2006.11.007

Figure 4. Activity of cathepsin P in cells and tissues.

Figure 4

Recombinant cathepsin P was assayed using MOCAc-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 in the presence or absence of E-64 (panel A). For comparison, cathepsin B and L activity were assayed using Cbz-Phe-Arg-N-methylcoumarin in the presence and absence of E-64 using pure enzymes (panel A). Activities are normalized to activity in the absence of inhibitor. Rcho-1 cells at different times after differentiation were homogenized in activity buffer and cathepsin P activity was determined in the absence (♦) and presence of E-64 (▲) and CtP-I (●) (panel B). Cathepsin P and B activities were measured in tissues from pregnant mice (panels C and D) and pregnant rats (panel E). In panels B, C, D and E, activities were calculated per mg protein and are normalized to tissues that exhibited maximal activity. Error bars indicate standard deviations and are derived from at least 3 separate samples.