Table 1.
Target | Primers, forward and reverse | Amplicon (bp) |
---|---|---|
cathepsin P (qPCR) | TGCAGTTGGTGCGATAGAAG | 110 |
GGCAGCCATTATTTCCTTCA | ||
cathepsin L (qPCR) | CAAAGACCGGAACAACCACT | 103 |
CCTTCGGATGTAGTGTCCGT | ||
β-actin (qPCR) | AGCCATGTACGTAGCCATCC | 115 |
ACCCTCATAGATGGGCACAG | ||
cathepsin P | TGAAGAGTTGAGTCTGTGGAGGACC | 526 |
GTTTTTGAGAACATCTGACC | ||
cathepsin L | TGTGGTTCTTGCTGGGCTTTTAG | 397 |
AACTGGAGAGACGGATGGCTTG | ||
cathepsin 1 | GGAGTGGAAGAGGAACAATGCG | 592 |
TGGGACAACAAAAAAGCGGG | ||
cathepsin 2 | ATGAAGGTCAATGCCTTTGG | 376 |
TTGACTCAGCCTCCAGACCT | ||
cathepsin M | CCTGGGCTGTTATTTAGGCA | 603 |
ACAACTGGCCTTCCAATGAC | ||
cathepsin R | CAGGACCTTGGGACATGACT | 603 |
TGCACACTCAGAGGGATGAG | ||
placental lactogen | CTCTGAAACACTTGGTCGTCTGC | 247 |
CGGCACAGGTTACAAATGGC | ||
β-actin | ACCCTCATAGATGGGCACAG | 600 |
AGCCATGTACGTAGCCATCC | ||
Mash2 | CTCGAGGACTTGTCGAGGAC | 528 |
GCAGCGTCTCCACCTTACTC | ||
Hand1 | CCTCAAGTCCGCAGGTC | 252 |
ACCAAAGAGGAAGGGTTCGT | ||
Stra13 | AACTTACAAATTGCCGCACC | 560 |
ACTGGCACACAGTTTTTCCC |