FIGURE 6.
CDK5 RNAi’s reduced phosphorylated Tau in control animals (littermates with only the PS1M146Vknock-in) but did not affect spatial memory. Morris Water Maze (MWM) testing was performed in 18-month-old control mice injected with shSCRmiR (white squares) and shCDK5miR (gray squares). (A) Values of latency to reach the visible and hidden platform during training and probe trial tests 48 h after training. (B) Time spent in the target quadrant and (C) path plots for the median path length during the probe trial. (TQ = target quadrant, RQ = right quadrant, UQ = upper quadrant and OQ = opposite quadrant.) Data are presented as mean ± SEM. n = 10 by each group. *p < 0.05. (D) Steady-state levels of CDK5, AT8 and AT100 decreased after injection of shCDK5miR in hippocampal lysates of aged littermate mice injected with shSCRmiR (white bars) and shCDK5miR (gray bars). There was no statistically significant difference between shSCRmiR and shCDK5miR in AT180, PHF1 and Tau5 levels. Representative blots are shown. Tubulin was used as a loading control. Densitometry quantification was done. RU = relative units. n = 4. *p < 0.05.