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. 2014 Sep 10;8:280. doi: 10.3389/fncel.2014.00280

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Copyright © 2014 Chuang, Huang and Hsueh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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T-brain-1 proteins are induced by neuronal activation in cultured neurons. (A–C) Double immunostaining with TBR1 and MAP2 antibodies. Primary amygdalar (A), cortical (B), and hippocampal (C) neurons were incubated with TTX (1 μM) or bicuculline (Bicu, 40 μM) for 6 h at 21 DIV. Neurons were then fixed for immunostaining. Scale bars: 20 μm. The bar graphs in right panels of (A–C) show the mean fluorescence intensity of TBR1 quantified by MetaMorph software. All data are presented as the mean + SEM. The number of neurons (n) analyzed for each experiment is indicated in the figure. ***P < 0.001.