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. 2014 Sep 10;8:280. doi: 10.3389/fncel.2014.00280

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Copyright © 2014 Chuang, Huang and Hsueh.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tbr1 upregulation correlates with Grin2b induction in cultured neurons. Quantitative-PCR analyses were performed to examine the expression of Tbr1 and Grin2b upon (A–C) TTX and bicuculline (Bicu) treatment and (D–F) NMDAR activation. Cultured amygdalar (A,D), cortical (B,E), and hippocampal (C,F) neurons were incubated with TTX (1 μM), bicuculline (40 μM), NMDA (10 μM), or vehicle (Ctrl) for 6 h. Immediately (0 h) or 12 more hours (12 h) after treatment, neurons were harvested for quantitative-PCR using Cyp as an internal control to quantify the relative mRNA levels of Tbr1 and Grin2b at 21 DIV. The experiments were performed independently at least three times. Data are presented as the mean ± SEM. *P < 0.05; **P < 0.001; ***P < 0.001; n.s., not significant.