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. 2014 Sep 10;8:280. doi: 10.3389/fncel.2014.00280

FIGURE 5.

FIGURE 5

Grin2b induction upon neuronal activation requires Tbr1 expression in neuronal cultures. Bicuculline (Bicu, 40 μM) was added to the WT and Tbr1 KO amygdalar cultures at 21 DIV for 6 h. Quantitative-PCR analysis was conducted to measure the relative mRNA levels of Tbr1 (A) and Grin2b (B) immediately (0 h) and 12 h after bicuculline stimulation. The Cyp expression level was used as an internal control. Tbr1 KO amygdalar neurons did not respond to bicuculline in terms of Grin2b induction. Experiments were repeated three independent times. Data represent the mean ± SEM. *P < 0.05; ***P < 0.001; n.s., not significant.