Figure 1.

Neurotoxicity of AAV-mediated aSyn expression in MDN culture. Neurons were transduced 4 h after plating with AAV vectors expressing EGFP (CTRL) or the aSyn variants (1 × 10⁸ transducing units/400,000 neurons/well) and were evaluated for cell survival after 6 days in culture. (A) Histogram showing percentages of surviving tyrosine hydroxylase (TH)-immunopositive neurons in comparison to the EGFP control. Bars represent means ± s.e.m. ^*p < 0.05, ^***p < 0.001. (B) Representative micrographs of MDN cultures labeled against TH (Cy3, red). Insets show additional DAPI nuclear counter-staining (blue). Scale bar = 400 μm, scale bar inset = 200 μm.