Figure 5.

Density of TH-immunopositive striatal fibers 12 weeks after AAV-mediated nigral aSyn expression and ipsilateral striatal 6-OHDA lesion. Nigral neurons were unilaterally infected with AAV vectors expressing EGFP (CTRL) or aSyn (WT, A30P and A53T form; each 1 × 10⁸ transducing units) and were simultaneously lesioned in the ipsilateral striatum with 6-OHDA (4 μg/2 μl). (A) Histogram showing the relative density of TH-immunopositive striatal fibers in comparison to the unlesioned striatum 12 weeks after lesion and AAV vector injection. Bars represent means ± s.e.m. n.s. = not significant; ^*p < 0.05, ^***p < 0.001. (B) Representative micrographs of the striatum and magnifications after immunostaining for TH (black). Black squares mark exemplary fields of fiber analysis. Scale bar = 500 μm (overview), 20 μm (magnification).