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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Nature. 2013 Nov 20;505(7481):103–107. doi: 10.1038/nature12729

Extended Data Figure 10. Modeling of hRegIIIα-membrane interactions.

Extended Data Figure 10

a, hRegIIIα pore complex model shown from the side. R166 is located near the water-membrane interface, suggesting that it is positioned to interact with the phospholipid headgroups, while R39 is predicted to be exposed to aqueous solvent. Membrane boundaries predicted from the computational calculations are indicated. b, 5 μM of wild-type, R166A mutant, or R39A mutant hRegIIIα was added to 100 μM CF-loaded liposomes and dye release was monitored. The experimental results are consistent with the position of these residues relative to the membrane interface in the model.