Skip to main content
. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Nature. 2013 Nov 20;505(7481):103–107. doi: 10.1038/nature12729

Extended Data Figure 8. hRegIIIα mutagenesis.

Extended Data Figure 8

a, Mutagenesis of K93 with conservative amino acid substitutions (R and H) does not alter membrane toxicity of hRegIIIα. 5 μM of wild-type, K93R mutant, or K93H mutant hRegIIIα was added to 100 μM CF-loaded liposomes and dye release was monitored. These mutants retain membrane toxicity, in contrast to K93A (Fig. 3e), suggesting the importance of positive charges at these sites. b, Filamentation of hRegIIIα mutants (K93A and E114Q) correlates with membrane toxicity. 20 μM hRegIIIα K93A (left panel) or E114Q (right panel) was incubated for 20 minutes with unilamellar vesicles and visualized by negative-staining EM. The results demonstrate that the non-toxic E114Q mutant, unlike the toxic K93A mutant, assembles into filaments.