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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Biochim Biophys Acta. 2013 Aug 13;1833(12):2970–2979. doi: 10.1016/j.bbamcr.2013.08.001

Fig. 5. ZBP-89 demethylates methyl-CpG dinucleotide.

Fig. 5

Schematic diagram of Bak promoter and ZBP-89 binding sites (A). ChIP experiment showed ZBP-89 binding to two regions. One is very close to exon 1 and the other is 3 kbp away form transcription start site (B). 204 bp S1 sequence which is 3 kbp away from exon1, and 228 bp S2 which is near exon1, were precipitated by ZBP-89 antibody and amplified by primers (C). MeCP2 binds to chromatin in a methylation-dependent manner and is associated with a core repressor complex to silence gene transcription. Results showed that adenovirus-mediated ZBP-89 overexpression decreased MeCP2 protein binding to chromatin (D). CpG islands in Bak gene promoter were predicted by online software and 2 potential CpG islands in Bak promoter were found (E). When ZBP-89 was overexpressed, some methylated CpG regions became demethylation (F).