Table 2. HILIC gradients.

Gradient	Step	Duration	Percent B
On-line WCX-HILIC Activation	Activate	30 min	99%
On-line WCX-HILIC Wash	Wash		1%
	Gradient	1 min	1%
		200 min	99%
Online WCX-HILIC H3(1-50) resolving gradient	Equilibrate	60 min	1%
	Gradient	1min	1%
		200 min	99%
Off-line HILIC resolving gradient	Equilibrate	15 min	1%
	Gradient	1min	1%
		60 min	99%
	Wash	5 min	100%