Table 3. MS settings.

	H3 Middle-Down	H4 Middle-down	Top Down
Spray Voltage	2.7	2.7	2.7
Source Voltage			?
FT-resolution	3.00E+04	30000	100,000
FT-Full MS-AGC	7.00E+05	7.00E+05	7.00E+05
FT-MSn-AGC	1.00E+06	1.00E+06	2.00E+05
IT-Full-AGC	5.00E+04	5.00E+04
IT-MSN-AGC	3.00E+04	3.00E+04
Maximum injection time (ms)	1.00E+02	1.00E+02	100
microscan number			10
Reagent ion AGC	3.00E+06	3.00E+06	1.00E+06
reaction time (ms)	100	100	5-7
isolation width (m/z)	2	2	3
Activation Q	0.25	0.25	0.25
mass range (m/z)	290-1200 590-650	393.0-440.0	start with 400 - 1500
Reject Charge states	+1, +2, +3	+1, +2, +3	NA
number of data dependent scans	7	7	NA
Exclusion width (m/z)	1	1	NA
Exclusion time (s)	60	60	NA
Lock Mass	371.101, 445.120	371.101, 445.121