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. 2014 Sep 3;55(9):5551–5560. doi: 10.1167/iovs.14-14580

Figure 5.

Figure 5

Downregulation of DJ-1 increases activated caspase3 and phospho-p53 levels by UV-A irradiation. (A) Cytotoxicity assay by measuring LDH release showed that cell death increased in DJ-1 siRNA-treated HECEi cells as compared to controls. Data were normalized with no UV-A treatment. (B) Representative Western blot of cleaved caspase3 protein levels. (C) Caspase3 activity assay by measuring fluorescence product of Z-DEVD-rhodamine 110. Data were normalized with protein concentrations. (D) Western blot analysis of phospho-p53 and total p53 (left panel), and densitometric analysis (right panel) of phospho-p53 protein levels in control siRNA-treated and DJ-1 siRNA-treated HCECi cells.