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. 2014 Aug 28;5:28. doi: 10.1186/2041-9139-5-28

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Copyright © 2014 Ma et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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αA-crys expression is controlled by trans-acting factors in F1 hybrid embryos. (A-D)In situ hybridization showing αA-crys expression in the lens of (A) surface fish (SF), (B) surface fish X cavefish (CF) F1 hybrids, and (C) cavefish X surface fish F1 hybrids, but not in (D) cavefish embryos at 72 hr post fertilization (hpf). The eye and lens are outlined by dashes in cavefish (D). Scale bar in A is 150 μm; magnifications are the same in A-D. (E) Semiquantitative RT-PCR showing αA-crys transcript levels in 40 hpf surface fish (SF), surface fish x cavefish F1 hybrid embryos (HY), and cavefish (CF) embryos compared to an 18S rRNA standard. (F) Profile of sequenced RT-PCR products from surface fish x cavefish F1 hybrid embryos showing a mixture of A (green) and G (black) residues at the first exon site distinguishing the surface fish from cavefish αA-crys alleles.