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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: J Pathol. 2009 Jan;217(1):113–121. doi: 10.1002/path.2463

Figure 4.

Figure 4

Suppression of PLA2G4B in decidual cells occurs through binding of miR-338 to PLA2G4B 3’UTR. (A) Target scanning of the PLA2G4B 3’UTR identified two putative miR-338 binding sites. The full-length PLA2G4B 3’UTR sequence was cloned in the downstream of the firefly luciferase gene in the reporter plasmid pMIR-REPORT™. (B) Transfection with the reporter plasmid containing the PLA2G4B 3’UTR decreased luciferase activity by 46% compared to a mock reporter plasmid. Renilla luciferase plasmid was used as a transfection control (n=3; p < 0.05). (C) Co-transfection of anti-miR-338 and a reporter plasmid with the PLA2G4B 3’UTR caused a 1.82 fold-increase in luciferase activity compared to cells co-transfected with anti-miR-338 and a mock reporter plasmid (n=3; p < 0.05).