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. 2014 Sep 10;9(9):e107291. doi: 10.1371/journal.pone.0107291

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© 2014 Soto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) To determine if the Akita mutation leads to RGC loss in DBA/2J mice, retinas from 8.5 month old D2.Ins2^Akita/+ and D2.Ins2^+/+ mice were stained for the RGC marker TUBB3+. (C) TUBB3+ cell counts showed that there was no significant difference in RGCs detected in retinas of D2.Ins2^Akita/+ mice compared with D2.Ins2^+/+ mice (n = 8 retinas for each genotype; p = 0.138). Scale bar: 30 µm.