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(A) One hundred pairs of SGH were loaded in a gel-filtration column, and eluted in PBS containing 0.1% PEG 4000. The fractions were tested for lectin activity. (B) MS analysis. The fraction (retention volume at 9 ml) containing the highest activity was used for 1D PAGE. Twenty gel bands were cut, followed by in-gel tryptic digestion, and nano-LC MS/MS (results presented in Table S1).
