Table 1. Specificity of An. gambiae SGH hemagglutinating activity.
Ligand | Inhibitory concentration* |
1. Sulfated polysacharides | |
Dextran sulfate 500,000 | <3.715 pM |
Dextran sulfate 50,000 | <37.15 pM |
Fucoidan | 470 pM |
Heparin Grade II | 78 µg/ml |
Condrotin sulfate A | NI up to 1.25 mM# |
Condrotin sulfate C | NI up to 1.25 mM |
Heparan sulfate | NI up to 0.25 mM |
Keratan sulfate | NI up to 0.25 mM |
Dextran 500,000 | NI up to 125 µM |
Dextran sulfate 5,000 | NI up to 1.25 mM |
2. Proteins | |
Laminin | 18 nM |
Heparan Sulphate Proteoglycan | 93 nM |
Glycophorin | NI up to 0.5 mg/ml |
Asialoglycophorin | NI up to 5 mg/ml |
Orosomucoid | NI up to 5 mg/ml |
Fetuin | NI up to 5 mg/ml |
3. Sialic acid-containing glycans | |
Sialic acid | 6.25 mM |
3′-sialyl Lewis X | 0.3125 mM |
3′-sialyl Lewis A | NI up to 1.25 mM |
3′-sulfated Lewis A | 0.625 mM |
3′-sialyl lactose | 0.156 mM |
6′-sialyl lactose | 0.078 mM |
Tetrasacharide A | NI up to 2.5 mM |
Tetrasacharide B | NI up to 2.5 mM |
Tetrasacharide C | NI up to 2.5 mM |
Di-sialyl lactose-tetraose | NI up to 2.5 mM |
3′-sialyl fucosil lactose | NI up to 2.5 mM |
4. Gangliosides | |
Monosialoganglioside (GM3) | 0.312 mM |
Disialoganglioside (GD1a) | 0.312 mM |
Disialoganglioside (GD1b) | 0.625 mM |
Trisialoganglioside (GT1B) | 0.156 mM |
Tetrasialoganglioside (GM1) | 0.625 mM |
Tetrasialoganglioside (GQ1B) | 0.2 mM |
5. Simple sugars | |
Maltose | NI up to 25 mM |
Galactose | NI up to 25 mM |
Trehalose | NI up to 25 mM |
Manose | NI up to 25 mM |
Glicose | NI up to 25 mM |
N-acetil-galactosamine | NI up to 25 mM |
*Inhibitory concentration; minimal concentration promoting inhibition of agglutination.
NI; non-inhibitory at the maximum tested concentration.