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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Biomaterials. 2012 Nov 3;34(4):912–921. doi: 10.1016/j.biomaterials.2012.10.020

Figure 5.

Figure 5

Effect of matrix rigidity, chemistry, and hydrophilicity on hPSCs. (a) Representative phase contrast images of adhered HUES9-Oct4-GFP cells on PSS-based hydrogels with varying bulk rigidity (top) and their corresponding fluorescence images (bottom). (b) Images of HUES9-Oct4-GFP cells on PSS-based hydrogels with varying mole ratio of acrylamide to sodium 4-vinyl benzene sulfonate (Am:SS; 6:0.5, 6:1, and 6:2); PAm6-co-PSS0.5, PAm6-co-PSS1, PAm6-co-PSS2 (top) (c) Representative phase contrast images of hPSCs grown on hydrogels containing different chemistries and hydrophilicities while maintaining identical sulfate functional groups and matrix rigidities. Scale bar: 200µm.