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. 2014 Sep 8;30(5):496–508. doi: 10.1016/j.devcel.2014.08.016

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© 2014 The Authors

This work is licensed under a Creative Commons Attribution 3.0 Unported License.

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The Mis18 Complex Licenses Chromatin for CENP-A Incorporation

During replication, CENP-A nucleosomes are divided between the two daughter DNA molecules and are either replaced with H3 (H3.1/H3.3) nucleosomes or leave nucleosome free gaps. During anaphase to telophase, the Mis18 complex localizes to centromeres. Soluble CENP-A-H4 binds to the chaperone HJURP before incorporation, and during early G1 the CENP-A-H4-HJUPRP complex is recruited into the licensed chromatin by binding to the Mis18 complex. Following CENP-A incorporation, H4K20 in CENP-A nucleosomes is monomethylated. This maturation step is required for certain of the constitutive centromere associated network (CCAN) proteins to assemble and subsequently direct assembly of a functional kinetochore.