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. Author manuscript; available in PMC: 2015 Oct 11.
Published in final edited form as: Chem Commun (Camb). 2014 Oct 11;50(79):11679–11682. doi: 10.1039/c4cc02969k

Table 1.

Reactivity of Palladacycles Carrying the Various Directing Groups toward Ub-Hpga

graphic file with name nihms622754u1.jpg
entry palladacycle conversion (%)b
10 s 3 min
1 graphic file with name nihms622754t1.jpg
1 		7 83 (3)c
2 graphic file with name nihms622754t2.jpg
2 		48 >99
3 graphic file with name nihms622754t3.jpg
3 		9 65 (25)c
4 graphic file with name nihms622754t4.jpg
4 		10 85 (10)c
5 graphic file with name nihms622754t5.jpg
5 		56 94 (6)c
6 graphic file with name nihms622754t6.jpg
6 		40 87 (11)c
7 graphic file with name nihms622754t7.jpg
7 		73 >99
8 graphic file with name nihms622754t8.jpg
8 		64 >99
a

Reaction was carried out by incubating 2.5 μM Ub-Hpg with 10 μM palladacycle at room temperature for the indicated time (see detail procedure in SI, general procedures for the reaction of palladacycles with Ub-Hpg).

b

Conversion was determined based on LC-MS analysis: conversion % = Iproduct/(IUb-Hpg + Iproduct + Idimer side product × 2), where IUb-Hpg, Iproduct and Idimer side product represent the ion counts of Ub-Hpg, product and the protein dimer side product, respectively.

c

Value in parenthesis denotes the conversion of the dimer side product. The mass of the dimer side product is consistent with the structure: Inline graphic; see ref. 25 for a possible mechanism.