Figure 1. Co-culture of ILC2s and CD4⁺ T cells enhances proliferation of CD4⁺ T cells and type 2 cytokine production.

(A) CFSE-labeled CD4⁺ T cells (2×10⁴ cells/well) were stimulated with anti-CD3/CD28 with or without isolated lung ILC2s (10⁴ cells/well) for 4 days. Cells were analyzed by FACS by gating on CD4⁺ T cells. Data are representative of three experiments. (B) CD4⁺ T cells (2×10⁴ cells/well) were stimulated with anti-CD3/CD28 with the indicated numbers of ILC2s for 4 days. Cytokine levels in the supernatants were analyzed by ELISA. *, p<0.05; **, p<0.01 as compared to no ILC2s in the culture. Data (mean±SEM, n=3) are representative of 2-3 experiments.