Figure 2. The interaction between ILC2s and CD4⁺ T cells is likely bidirectional.

(A) CD4⁺ T cells (5×10⁵ cells/well) and ILC2s (5×10⁵ cells/well) were cultured alone or together for 20 h and then sorted by FACS. Cytokine mRNA expression in each cell population was analyzed. Data are presented as the ratio of mRNA copy numbers in co-cultured cells divided by mRNA copy numbers in cells cultured alone. Data are from three experiments. (B) CD4⁺ T cells derived from WT or Il5^−/− mice were stimulated with anti-CD3/CD28 with or without ILC2s from WT mice for 4 days. *, p<0.05; **, p<0.01 between the groups indicated by horizontal lines. Data (mean±SEM, n=3) are representative of two experiments.