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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Pathog Dis. 2014 May 8;72(1):10–18. doi: 10.1111/2049-632X.12167

Fig. 6.

Fig. 6

IglE interacts with the PdpB C-terminus. (a) Escherichia coli reporter strain KDZif1ΔZ was transformed with either empty vectors pACTR-AP-Zif and/or pBRGPω (Karna et al., 2010), plasmids pKEK1613 (PdpB590–1093-Zif), pKEK1254 (IglE23–125-ω), pKEK1416 (IglA-Zif), and pKEK1415 (IglB-ω), and assayed for beta-galactosidase activity. All values are result of triplicate samples, **P < 0.01. (b) Whole-cell lysates from Francisella tularensis ssp. novicida strain KKF194 (ΔiglE) or KKF219 (ΔFPI) either with no plasmid (‘–’) or carrying pKEK1355 (expresses IglE-FLAG) were subjected to immunoprecipitation as described in Materials & Methods with α-FLAG. Samples were separated by PAGE and subjected to Western immunoblot analysis with anti-PdpB or anti-FLAG antibodies.