Figure 3. Strains lacking sitA or ΔmntH do not limit replication in mice or macrophages.
Mice were orogastrically inoculated with WT, ΔsitA or ΔmntH strains. Fecal pellets were harvested at 1 and 3 days post-infection (A). Tissues were harvested and plated for CFU at 2 weeks post-infection (B, C). Mean and SEM are shown. p-values were determined as described in the methods. p < 0.05 (*) vs. WT. Each symbol represents one mouse, n = 5. D) RAW264.7 Nramp1+ cells were treated with IFNγ and LPS and inoculated with the strains indicated at an MOI of 10. Mean and SD of a representative experiment are shown. n ≥ 3 experiments.