Skip to main content
. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Mol Microbiol. 2014 Aug 14;93(6):1314–1326. doi: 10.1111/mmi.12739

Figure 4. Increased replication in the absence of feoB is dependent on ferric iron uptake.

Figure 4

A) RAW264.7 Nramp1+ cells were infected with WT or ΔfeoB mutant strains at an MOI of 10. At 2 hours post-infection, macrophages were lysed and isolated RNA was subjected to real-time RT-PCR. Agarose gel, left, quantification, right. fepB amplicon, 245bp and 16s amplicon, 850bp. Mean and SEM are shown. p < 0.05 (*), n = 3 experiments. B) IFNγ and LPS activated RAW264.7 Nramp1+ cells were inoculated with the strains indicated at an MOI of 10. Mean and SD of a representative experiment is shown. p < 0.05 (*), n ≥ 3 experiments. C) Mice were orogastrically inoculated with WT or ΔiroN, ΔfepA, ΔfeoB strains. Tissues were harvested and plated for CFU at 2 weeks post-infection. Mean and SEM are shown. Each symbol represents one mouse, n ≥ 5. p-values for all experiments were determined as described in the methods. D) Comparison of colonization of the spleen and liver by the indicated strains. P < 0.05 (*)