Figure 5. In mixed infections of mice, FeoB is required for S. Typhimurium replication.
Mice were orogastrically inoculated with a 1:1 mixture of WT and ΔfeoB strains (A-C), WT and ΔmntH strains (D), or WT and ΔsitA strains (E). Fecal pellets were harvested 1 and 3 days post-infection (A). Tissues from SV129S6 (B) or C57BL6 Nramp1G169 (C-E) mice were harvested and plated for CFU at 2 weeks post-infection. Mean and SEM of competitive index are shown; dashed lines indicate equivalent competition. p-values were determined as described in the methods. p < 0.03 (**) vs. the null hypothesis. Each symbol represents one mouse, n = 4-5.