Table 1.
Summary of different feeding modes and their effect on P4HB production during fed-batch cultivations
| Growth substrate/stimulator/precursor/media | Feeding strategy | Culture time (h) | OD 600max | CDW (g L −1 ) | P4HB content% (w w −1 ) | Volumetric yield P4HB (g L −1 ) | Productivity (g L −1 h −1 ) | References |
|---|---|---|---|---|---|---|---|---|
| Glycerol/acetate/Na-4HB/modified M9 medium | Pulse feed | 64 | 77.9 | 42.8 | 26 | 11.5 | 0.180 | This study |
| Linear feed | 118 | 63.8 | 33.8 | 30 | 6.8 | 0.058 | ||
| Exponential feed | 73 | 81.5 | 43.2 | 33 | 15.0 | 0.207 | ||
| Glucose/-/4HB/M9 medium | Pulse feed | 62 | 24.5 | 13.0 | 31 | 4.0 | 0.065 | [31] |
| Glucose/-/yeast extract/LB medium | Pulse feed | 52 | 21.7 | 11.5 | 68 | 7.8 | 0.150 | [32] |